PJB-2018-42
Candidate genes involved in the nectar produce in pepper flower are identified by transcriptome analysis
minghua deng
Abstract
The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different development stages of the pepper (Capsicum annuum L.) flower (stage 1, sporogenous cell division, stage (B1); stage 2, pollen mother cell meiosis, stage (B2); and stage 3, open flower (B3)) to measure gene expression. In the cDNA libraries for B1, B2, and B3, approximately 53, 55, and 55 million clean reads were assembled; 147640, 127553, and 165374 contigs were obtained; and 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34445 unigene sequences and 128 pathways were annotated by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified in the flower, and 8955, 12182, and 23667 differentially expressed genes (DEGs) were identified in the B2 vs B1, B3 vs B1, and B3 vs B2 comparisons. KEGG pathway analysis demonstrated that DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. All of the 13 selected DEGs exhibited similar expression patterns after quantitative real-time PCR analysis according to the RNA-seq data. Sucrose-phosphatase, galactinol--sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The newly generated transcriptome and gene expression profiling data provide valuable genetic information that reveals the transcriptomic variation during flower development in pepper.
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