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RAPD based characterization of chickpea isolates of Sclerotium rolfsii

Abida Akram, Pakeeza Amber, Sheikh Muhammad Iqbal, Rahmatullah Qureshi, Arshad Javaid and Saeed Mukhtar

Collar rot disease caused by the fungus Sclerotium rolfsii Sacc., results in heavy economic losses to chickpea (Cicer arietinum L.) crop every year. Previous work on this devastating fungus reported just its morphological and physiological aspects, but little is known at molecular level. Present research was focused on estimating the genetic variation among 12 strains of S. rolfsii isolated from infected chickpea plants through Random Amplification of Polymorphic DNA (RAPD) analysis. Fungal strains were obtained from Crop Sciences Institute, National Agricultural Research Centre (NARC), Islamabad. Out of 40 random decamer primers studied, 6 gave the clear polymorphic amplification pattern in terms of band number. A total of 52 loci were traced by these primers and 100% polymorphism was obtained. The value of similarity matrix ranged from 55.8–88.5%. Strain AM-04 and AM-06 shared maximum of 88.5% similarity while AM-07 and AM-09 were 55.8% similar. Similarity of the remaining genotypes was between 55.7–84.6%. Cluster composition results indicated that AM-07 was the most diverse from the rest of genotypes which showing genetic distance of 35.7%. On the whole, all the strains were 64.3% similar to each other and only 35.7% variation existed among them

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