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Mycoflora associated with raisins (Vitis vinifera L.) collected across Pakistan

Shahnaz Dawar, Marium Tariq and Shaista Aslam

Aim of study was to isolate and identify fungal pathogens associated with raisins (Vitis vinifera L.) collected from Punjab, Sindh, Baluchistan, Khyber Pakhtunkhwa provinces of Pakistan. Around 25 fungal species belonging to 15 genera were isolated from the fifteen samples by using ISTA (International Seed Testing Association) techniques. Of these, 19 species belonging to 11 genera were isolated by deep freezing method, agar plate method yielded 16 species belonging to 9 genera and 16 species belonging to 11 genera were isolated by blotter method. Deep freezing method was found best for isolation of fungi followed by standard blotter method. Species of Aspergillus and Penicillium were the most dominant fungi. Samples of raisins from the areas of Lahore, Islamabad and Karachi, respectively were found to be highly infected with fungi. These samples were treated with Ultra Violet (UV-C) radiations which significantly affected the pathogenic profile, but the conidia of Aspergillus niger were appeared to be more persistent that colonized the raisins within the storage time of zero day. However, infection by other storage fungus like Aspergillus oryzae (Ahlburg) Cohn. was observed after the storage time of 30 and 60 days. 

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