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Identification of candidate proteins and networks related to salinity stress in shrub willow roots by comparative proteomic analysis

Sui Dezong, Wang Baosong, Xu Lian and Shi Shizheng

Salinity stress is one of the major abiotic stresses that limit plant growth. To understand the mechanism of shrub willow clones roots in response to salt stress and explore candidate proteins for plant breeding associated with salt stress, the total proteins from seedling roots of salt-sensitive cultivar JW9-6 and salt-tolerant cultivar JW2372 under salt stress for 2, 12 and 72 h, respectively, were analyzed by 2-D electrophoresis. Finally, 109 differentially expressed proteins were successfully identified by MALDI-TOF/TOF MS. By analyzing and camparing functions of the differentially expressed proteins, obtaining the conclusions as follows: 1) the majority functions including BP (metabolic process and protein folding), CC (cytoplasm and cell) and MF (cofactor, coenzyme binding and isomerase activity), regulated by root proteins, were differences between JW9-6 (salt-sensitive) and JW2372 (salt-tolerance) under salt stress; 2) six pathways were changed by salt stress, including pyruvate metabolism, glycolysis, ascorbate and aldarate metabolism, amino sugar and nucleotide sugar metabolism, pentose phosphate pathway, and cysteine and methionine metabolism; 3) the five proteins including glucose-6-phosphate isomerase, ATP synthase epsilon chain, phosphoglycerate kinase, S-adenosylmethionine synthase 4 and adenosine kinase, could be candidate proteins for plant breeding associated with salt stress; 4) differences from the salt-responsive pathways between leaves and roots in the shrub willow clones could provide an important strategy for forest tree breeding involved in salt tolerance.

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