PJB-2017-658
Genetic Variation and Phylogenetic Relationship among Different Leaf Lettuce (Lactuca sativa L.) Varieties in Korea
Sun Yan-Lin
Abstract
The genus Lactuca, known as vegetables or oil crops, is widely cultivated in Canada, Australia, European and Asian countries. Many various lettuce vegetables are just included in this genus. To satisfy people’s demands of nutrition and taste on lettuces, abundant germplasm and hybrids have been bred out by breeding programs. This results in difficult variety identification of these germplasm and hybrids, that would baffle further trait improvement and target selection. In this study, we investigated 18 distinguished edible lettuce varieties, and evaluated their genetic divergence and phylogenetic relationship in molecular level. DNA alignment results suggest that the rRNA 5S gene was the most variable marker among investigated markers, and the nrDNA ITS gene was not sensitive between Lactuca and Brassica genera. S3 and S5 just showed two nucleotide array deletions and one addition as comparison to other lettuces. In the 5S phylogenetic tree, materials were divided into two groups, and S3 and S5 showed nearer phylogenetic relationship. The grouping was considered to relate with leaf color but not leaf shape. However, seen from the phylogenetic tree constructed by combined gene sequences, it further proved the relations between grouping and leaf color and shape. This work could help clear variety discrimination and target selection for lettuce breeding programs.
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