PJB-2017-618
PHYTOCHEMICAL, ANTIOXIDANT, IN VITRO ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF WITHANIA SOMNIFERA (L.) DUNAL.USED IN TRADITIONAL MEDICINE IN PAKISTAN
Gul Jan
Abstract
The present study was aimed to evaluate the phytochemical, antioxidant, antimicrobial and cytotoxic activities of ethanolic extract of Withania somnifera (L) Dunal. The ethanolic extract of W. somnifera leaves contains flavonoids, alkaloids, saponins and tannins and also showed significant DPPH free radical scavenging activity with the IC50 value of 16.62 μg/ml. Reducing power of W. somnifera was also moderately stronger. These activities were confirmed by in vitro cytotoxic studies in which W. somnifera showed the maximum control over HeLa cell lines with the IC50 value of 40 μg/ml. Ethanolic extracts were used against infectious pathogenic bacteria i.e. three Gram-positive bacteria (Staphylococcus aereus, Bacillus subtlilis and Bacillus atropeous) and four Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsela pneumonia and Salmonella typhi). In vitro antimicrobial activity was determined by disc diffusion method. The activity was expressed as mg/µl. Ethanolic extracts of W. somnifera was found active against all microbes. Further studies are needed for establishing safety, toxicity and pharmacological activity with the phytochemical investigation. This research supports the uses of the selected medicinal plant against bacterial infection. Key words: Phytochemical; antioxidant; In vitro Antimicrobial activity and cytotoxicity; Withania somnifera
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