PJB-2017-587
QTL analysis of wheat FHB resistance at different developmental stages
Le Chang
Abstract
In order to reveal the genetic basis of wheat FHB resistance a recombinant inbreed line (RIL) population including 160 plants derived from a cross between PI277012 and Jinmai47 was used in this study. Wheat recombinant inbred line (RIL) population was inoculated in a suspension of Fusariumgraminearum spores and cultured indoor, during which pathogenesis was observed. Disease index and area under the disease progress curve (AUDPC) were calculated on days 5, 10, and 15 to analyze the dynamic changes in wheat FHB resistance at different developmental stages. During the first stage, no QTL (quantitative trait locus) was found, and disease resistance index of each strain increased steadily and uniformly. We speculated that Fusariumgraminearum expanded in wheat spikelet when the wheat plant has yet to respond, resulting in a small difference in disease resistance between the different lines. During the second stage, four loci with additive effects were found, which were mapped to chromosomes1A, 1D, 3B and 4D,respectively,with QTLs located between wmc777 and barc147onchromosome 3B, which was consistent with previous results. We speculated that the large differences in resistance index were attributed to the QTLs controlling wheat resistance, leading to differences among different wheat strains. During the third stage, three QTLs were located on chromosomes1A, 5A and 7A, respectively. Compared with the traditional field cultured inoculation, field topsoil inoculation or natural breeding, the indoor experiment was highly controlled, facilitating annual comparison independent of variations in climate.
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