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IN VITRO PROPAGATION OF GLOXINIA (SINNINGIA SPECIOSA)
Abstract
Parts of newly formed leaves of Gloxinia were used as an explant and cultured on Murashiage and 5koog (1962) media containing different concentration of auxin and cytokinins. Shoot was induced from leaf explant on MS medium supplemented with 3 mg/l BAP. Shoot initiation generally started within 70-78 days after the formation of shoot primordia. These newly formed shoots were transferred to MS medium with 2mg/l BAP for further multiplication. Micro cuttings of 2-2.5cm in length were harvested trom proliferating cultures and transferred to root induction media containing different doses of NAA and IBA. Plantlets grown in this medium for 4-5 weeks could be successfully established in sterile sandy soil following hardening phase of 20 days and nourishment with Hoagland solution
