PJB-2011-414
ASSESSMENT OF ANTIBACTERIAL ACTIVITY OF IN VITRO AND IN VIVO GROWN GARLIC (ALLIUM SATIVUM L.)
ANEELA FATIMA,* TAUQEER AHMAD, SHAISTA J. KHAN, FARAH DEEBA AND NASREEN ZAIDI
Abstract
Antibacterial activities of In vitro and In vivo grown garlic were compared against five bacterial strains Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter aerogenes and Staphylococcus aureus. In 8-9 weeks, In vitro garlic bulblets were produced from shoot tip explants callus of garlic cloves cultured on modified MS medium. Ten µM BA induced somatic embryogenesis in soft, granular and dirty white to yellow colored clumps of calli, regenerated into plantlets, which eventually transformed into 9 or 10 mm dia bulblets on basal MS medium. Clear zones of inhibition were demarked by paper disc diffusion method. The content of micro-bulblets expressed greater antimicrobial activity than that of In vivo garlic cloves through wider diameter zone of inhibition against said bacterial strains. In vitro garlic extract formed 24mm and 22mm zones being the widest zones against Klebsiella and Proteus respectively.
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