PJB-2011-389
BIO-REACTIVE PROPERTIES OF CITRUS WASTE: AN INVESTIGATION OF ANTIOXIDANT AND TYROSINASE INHIBITORY ACTIVITIES
MUSARRAT AKHTAR1, LUBNA IQBAL2*, MEHREEN LATEEF2, BUSHRA NAWAB1, MUHAMMAD SALEEM2 AND NIGHAT AFZA1
Abstract
The antioxidant potential as well as tyrosinase inhibitory activity of citrus fruit waste were determined. Fruit peels of 4 species of genus citrus viz. Citrus sinensis (Malta), C. reticulate (Orange), C. paradisi (Grape fruit) and C. aurantifolia (Lemon), were analyzed for these bio-reactive properties. Six different fractions of crude extracts of each citrus fruit peel were screened for antioxidant behavior through DPPH scavenging activity, while tyrosinase inhibition capacity was evaluated by using kojic acid as standard tyrosinase inhibitor. It was revealed that significant antioxidant potential was found in chloroform fraction of malta, orange and lemon, while chloroform and ethyl acetate, both fractions of grape fruit had 90% antioxidant activity at 1% concentration. Alcoholic fractions of orange, malta, and lemon showed 90, 87, and 69% tyrosinase inhibition respectively, while no tyrosinase inhibition was found in any fraction of grape fruit.
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