PJB-2025-179
GENOME-WIDE IDENTIFICATION, CHARACTERIZATION, AND EXPRESSION ANALYSIS OF THE SKP1.1 FAM
Junjie Wang
Abstract
Abstract
SKP1 (S-phase Kinase-associated Protein 1) functions as an adaptor protein connecting F-box proteins to Cullin1 in the SKP1-Cullin1-F-box (SCF) ubiquitin ligase complex, which performs essential functions in response to abiotic stresses. However, the specific roles of SKP1 in plant responses to tailings dam water stress remain unclear. This study identified 30 SKP1.1 family members in Nicotiana benthamiana through bioinformatics analysis. The expression patterns of NbSKP1.1 were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) when plants were subjected to tailings dam water stress. Results demonstrated that exposure to tailings dam water pollutants suppressed the expression of NbSKP1.1. Confocal imaging revealed that NbSKP1s (NbSKP1.1, NbSKP1.2, NbSKP1.3, and NbSKP1L1) exhibit dual localization in the nucleus and cytoplasm. This comprehensive analysis of the NbSKP1.1 family in plants establishes a foundation for future investigations into plant-environment interactions.
Keywords: Nicotiana benthamiana; Tailings dam wastewater; NbSKP1.1; Bioinformatics analysis; Subcellular localization.
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