PJB-2025-136
Comparative phytochemical profiling of Soybean (Glycine max (L.) Merr. seed extract using GC-MS and in silico identification of beta catenin inhibitory phytocompound for cervical cancer treatment
Sadia Haleema
Abstract
Metabolomics aims to provide a comprehensive and unbiased analysis of all metabolites and is therefore a very powerful tool for characterizing the plant metabolome and is of great interest to plant scientists. Because of the diversity of structural classes of metabolites, there is no single methodology that can measure the complete metabolome in one step. By employing a combination of different instrument platforms and techniques, the differences in the metabolite profiles can be revealed. Cervical Cancer is the most lethal gynecological malignancy and is leading cause of cancer death in females. Wnt signaling is an evolutionarily conserved regulatory pathway that governs numerous normal cellular and developmental processes such as cell fate determination, cell proliferation and migration. However, aberrant Wnt signaling has also been identified as a key mechanism in cancer biology. ß -catenin is the key mediator of the Canonical Wnt pathway. In the absence of a Wnt ligand, ß-catenin is degraded by a destruction complex. Current work provides a complete metabolite analysis of polarity based extracts of Glycine max (L.) Merr) seeds via GC-MS analysis, followed by in silico identfication of only one compound which has been further quantified by UV-Vis. Spectroscopy. Results showed that all extracts contained multiple compounds, predominantly 9,12-Octadecadienoic acid (Z,Z)-, methyl ester, 12-Octadecenoic acid, methyl ester, 11-Octadecenoic acid, methyl ester, Linoleic acid ethyl ester, (E)-9-Octadecenoic acid ethyl ester and 4',7-Dihydroxyisoflavone Daidzeol (whose retention time varied from 13. 797 min 9.097 min). In-silico study showed that daidzein from G. max (L.) Merr. has maximum binding affinity with beta catenin, which may be ultimately inhibited followed by the inactivation of Wnt/β -Catenin pathway. Spectrophotometric analysis showed that ethanolic extract of plant had highest concnentration of 4',7-Dihydroxyisoflavone Daidzeol. So this compound can be isolated from the active extract and can be used for the evaluation of therapeutic effects via in vitro and in vivo studies.
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