PJB-2025-84
SPECTROSCOPIC AND MICROSCOPIC ANALYSIS OF POLYPHENOLICS, ANTIOXIDANT, ANTIMICROBIAL, AND DENTAL EROSIVE POTENTIAL OF PUNICA GRANATUM (POMEGRANATE)
Rehana Masood
Abstract
Abstract
The supreme mineralized tissue of human body is tooth enamel, which consists of 96% inorganic and 4% organic materials along with water while dentine consists of 70% inorganic materials. In the current study, Punica granatum (pomegranate) was characterized for various phytochemicals by chemical colourimetric/precipitation reactions, UV-Visible, FT-IR, and NMR spectroscopic techniques. In phytochemical analysis, P. granatum extracts showed the presence of glycosides, glucosidal saponins, terpenoids, and flavonoids. Furthermore, the total phenolic and flavonoids content (TPC and TFC) quantified in P. granatum extracts showed that ethyl acetate (428.9 mg GAE/g), acetone (323.8 mg GAE/g), and methanol (326.2 mg GAE/g) extracts are rich sources of polyphenolic compounds. The extracts were also significant inhibitors of DPPH free radical (70.3 to 119.2 µg/mL) and bacterial strains (7.00 to 18.5 mm). The aqueous extract of P. granatum, carbonated soft drink, and a mixture of both were used to determine its erosive effect on teeth via gravimetric analysis. The results of erosive study showed that the P. granatum aqueous extract (AE-PG) and the carbonated soft drink reduced the weight of teeth samples while a mixture of both caused very slow teeth weight reduction thus revealing that AE-PG protects teeth against carbonated drink decay. The results of scanning electron microscopy (SEM) further confirmed teeth degradation by the subject analysed solutions. Hence, the study concluded that AE-PG itself causes teeth degradation but protects the teeth decay by carbonated drinks.
Keywords: Punica granatum (pomegranate), polyphenolics, antioxidant, antimicrobial, dental erosive potentials
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