PJB-2024-581
BIOCHEMICAL ANALYSIS AND ACTIVITY PROFILING OF SUCROSE METABOLIZING ENZYMES OF FEMALE AND HERMAPHRODITE PAPAYA PLANT FRUIT AT DIFFERENT PHENOLOGICAL STAGES
Zunaira Riaz
Abstract
Papaya (Carica papaya L) is also called nutraceutical or functional fruit due to its extraordinary nutritional and medicinal properties. In this study we engendered a comparative profile of female and hermaphrodite papaya plant fruits at three phenological stages (mature, intermediate and green). Physical parameters of fruit and seed were estimated. Biochemical analyses of (carbohydrate, protein and reducing sugar) concentrations were investigated. Organoleptic preference of fruits was evaluated. Enzymatic profiles of Sucrose synthase (SS), sucrose phosphate synthase (SPS) and Acid invertase (AI) were analyzed in three maturity stages of both type of papaya plant fruit. Varying response of organoleptic evaluation were noticed. Both types of fruits exhibited acceptable organoleptic scores at maturity level. The panelists describe female papaya fruit in terms of aroma, flavor and overall acceptability, and ranked them in order of preference on hermaphrodite plant fruit. Female plant fruit is predominantly characterized by high protein content, reducing sugar content and carbohydrate content. Fruits of hermaphrodite and female plants exhibited evidently high level of AI in mature stage of fruits, and decreased activity was found in young fruit. In contrast, SS activity was found high in green stage and declines with the fruit senescence. However constant activity of SPS was noticed in both types of fruit. Thus, it can be concluded that AI and SS enzyme activities are responsible of sugar accumulation during ripening stage. These two significant enzymes participated in the regulation of fruit sink strength during the developmental period. These novel approaches provide the quality-based indexing of papaya fruit. This quality profile will contribute to an integrated picture for consumers in the selection of fruits appropriate to their intended use.
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