PJB-2024-522
EFFICACY OF BEAUVERIA BASSIANA AND METARHIZIUM ANISOPLIAE FOR THE MANAGEMENT OF TOMATO FRUIT WORM, HELIC
Abdul Basit
Abstract
The present experiment was conducted to evaluate the effect of Beauveria bassiana and Metarhizium anisopliae against tomato fruit worm, Helicoverpa armigera under controlled laboratory conditions i.e., 25±2°C temperature and 65±5% R.H. with 08:16 hrs. (L:D) photoperiod. Three different concentration viz., 1x108, 5x107 and 1x107 spores/ ml of tested entomopathogenic fungi i.e., B. bassiana and M. anisopliae were prepared in the laboratory and inoculated on H. armigera larvae by using micro-pipette. In other experiment, tomato nursery was planted in pots and prepared concentrations (1x108, 5x107 and 1x107 spores/ ml) of above mentioned entomopathogenic fungi were sprayed on tomato plants (5-7 leave stage) including control (water) by using suitable hand pump sprayer. The treated plants were shifted in insect cages and two pairs of H. armigera adults were released in each cage for egg laying. Both experiments were conducted under Completely Randomized Design (CRD) with three replicates. Results showed that earliest mean mortality of H. armigera i.e., 3.33 days was recorded in B. bassiana @ 1 x 108 spores/ml while late mortality was recorded in control treatment i.e., 15.67 days. The minimum corrected mortality of H. armigera i.e., 26.80% was recorded in M. anisopliae @ 1 × 107 spores/ml while the maximum mean corrected mortality was recorded in B. bassiana @ 1 × 108 spores/ml i.e., 86.86%. In addition, the minimum pupae recovery (%) i.e., 6.67%, pupae weight (0.19 gm) and no adult emergence of H. armigera was also recorded in B. bassiana @ 1 × 108 spores/ml as compared to other treatments. Probit analysis showed that the LC50 recorded for B. bassiana and M. anisopliae was 1.721 x 107 and 3.966 x 107 spores/ ml respectively while LT50 recorded for B. bassiana and M. anisopliae was 10.230 and 10.496 days respectively. The maximum ovipositional preference (fecundity) and egg hatching (%) was recorded in control treatment i.e., 103.33 eggs and 86.30% respectively while minimum egg laying and egg hatching was recorded in B. bassiana @ 1 × 108 spores/ml i.e., 21.00 eggs and 49.58% respectively. Damage assessment and yield data showed that treatment, B. bassiana @ 1 × 108 spores/ml gave good results with minimum mean damage assessment and maximum yield increase over control treatment i.e., 17.58% and 54.40% respectively. It is concluded that B. bassiana and M. anisopliae at high concentration (1x108 spores/ ml) could be used for the management of tomato fruit worm, H. armigera in vegetable crop.
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