PJB-2024-184
IN VITRO ANTICANCER AND ANTAGONISTIC POTENTIAL OF PHYTO-SYNTHESIZED CITRATE COATED ZINC OXIDE NANOPARTICLES
KAINAT TARIQ
Abstract
Background-Zinc oxide (ZnO) nanomaterials have become widely used metal oxide in medical research primarily for the production of antibacterial and anticancer medications due to their distinctive physiochemical properties. Objectives-The purpose of this investigation was to evaluate the efficiency of citrate coated zinc oxide nanoparticles (Cit-ZnO) NPs as well as check their antagonistic and anticancer application through in vitro assay. Results- The findings of UV-visible spectroscopy indicated an absorbance peak at a frequency of 280 nm which confirms the formation of nanoparticles. Scanning electron microscopy analysis demonstrated the spherical shape of nanoparticles with size ranges from 56 nm to 145 nm. X-Ray Diffraction analysis results indicated that nanoparticles have a good crystalline structure. The average grain size for all the peaks has been measured using Scherrer’s equation which ranges from 27 nm to 133 nm. The in vitro cytotoxic results illustrated that the growth of HePG2 cells were inhibited with increased concentration of (Cit-ZnO) NPs. Agar well diffusion assay was performed to evaluate the antagonistic effect of (Cit-ZnO) NPs against six bacterial strains including gram-positive (Staphyllococcus aureus, Listeria monocytogenes, Streptococcus pneumonie, and Streptococcus mutans) and gram negative (Escherichia coli and Pseudomonas aeuriginosa). Conclusion-The results revealed that (Cit-ZnO) NPs entirely inhibit the growth of pathogens at a concentration of 25µg/ml.
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