PJB-2024-167
ENHANCING SEED GERMINATION AND SEEDLING GROWTH OF ENDANGERED INDONESIAN ORCHID DENDROBIUM LINEALE ROLFE WITH SUCROSE AND COCONUT WATER SUPPLEMENTED
Edy Setiti Wida Utami
Abstract
Dendrobium lineale Rolfe, primarily discovered in Papua, Indonesia, is an important orchid species used as a parent in breeding. In addition, D. lineale also prosses medicinal attributes for anti-cancer properties. Currently, this orchid is classified as an endangered species and is included in Appendix II according to CITES. In this case, In vitro orchid seed culture aids conservation by boosting genetic diversity in endemic threatened plants. Therefore, this study aimed to investigate the in vitro effect of sucrose on seed germination and protocorm development. To achieve this, Vacin and Went (VW) medium was supplemented with varying concentrations of sucrose, namely 0, 10, 20, and 30 g/L. The effect of coconut water (CW) at concentrations of 0, 5, 10, 15, and 20% on root induction and seedling development was examined. The result showed that sucrose supplementation significantly enhanced seed germination and protocorm development in D. lineale. The inclusion of CW in the VW medium had a significant impact on root induction and seedling development. Additionally, it was discovered that the addition of 20 g/L of sucrose yielded the highest seed germination rate of 89.0% and protocorm with the first and second leaves at 46.0%. Among the different levels of CW tested, 20% exhibited superior outcomes in terms of seedling ¬rooting, enhanced number of leaves, and dry weight of plantlets, and none of the plantlets underwent necrosis.
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