PJB-2024-139
Antimicrobial properties of some plant extracts against resistant pathogens.
maqsood khan
Abstract
Abstract
AMR is a serious issue worldwide, especially in less developed countries. South-Asia is deliberated to be the central region for antibiotic-resistant bacteria. It is anticipated that 70% of antibiotic resistance is ascending in the Asia region, making it county-wide and worldwide hazard . Pakistan is a developing country of the South-Asia, rich in antibiotic resistance, a significant global and regional threat.The objective of this study was to determine resistance pattern of plant extracts against resistant pathogen.
Method
Disc diffusion method is applying to evaluate action of plant extracts on bacteria, hence this method was applied for comparison of effects of various jasmin leaves and jasmin flower as well as onion extracts concentration, which was attained by solvents water and methanol. There were 4 bacterial strains used to investigate antibacterial potency of individual plant. The microbes were proven to be resistant pathogens. One strain of Gram positive (S. aureus and three strains of Gram negative (E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) bacteria were used. Media specific for each strain were prepared (MSA for staphylococcus aureus, and MacConkey for Escherichia coli and klebsiella pneumonaie and nutrient agar for pseudomonas aerogenosa ) and on each labeled plate that contains the medium, was inoculated 40 μl of standardized broth culture of the bacteria.
Results
in pure form concentration plant leaves extracts of jasmin leaves shows 13mm zone of inhibition against Escherichia coli and against Methicillin Resistant staphylococcus aureus (MRSA) shows 6mm zone of inhibition.Against Klebsiella pneumonaie shows 6mm zone of inhibition.Against pseudomonas aerogenosa shows no zone of inhibition and labelled as resistant. In pure form concentration of plant leaves extracts of jasmin flower shows zone of inhibition 10mm against E.coli and shows 16mm zone of inhibition against Klebsiella pneumonaie.no zone of inhibition was found against methicillin resistant staphylococcus aureus and pseudomonas aerogenosa and labeled as resistant. Plant part of leaves extracts of Alium cepa(onion)showed no zone of inhibition against all resistant pathogens.
Conclusion
Antimicrobial resistance is alarming issue globally need to combat this issue. Our study shows that Jasmin leaves and jasmin flower and plant part of leaves extracts of Alium cepa (onion) showed no zone of inhibition against most of the resistant pathogen used in this study.But significant zone of inhibition was found aginst E.Coli, Klebsiella pneumonia.Similar studies need to conduct on resistant pathogen using different plat parts extract to analyse resistance patten of plant parts extracts against resistant pathogen so that better alternative should be detected.
Abstract
AMR is a serious issue worldwide, especially in less developed countries. South-Asia is deliberated to be the central region for antibiotic-resistant bacteria. It is anticipated that 70% of antibiotic resistance is ascending in the Asia region, making it county-wide and worldwide hazard . Pakistan is a developing country of the South-Asia, rich in antibiotic resistance, a significant global and regional threat.The objective of this study was to determine resistance pattern of plant extracts against resistant pathogen.
Method
Disc diffusion method is applying to evaluate action of plant extracts on bacteria, hence this method was applied for comparison of effects of various jasmin leaves and jasmin flower as well as onion extracts concentration, which was attained by solvents water and methanol. There were 4 bacterial strains used to investigate antibacterial potency of individual plant. The microbes were proven to be resistant pathogens. One strain of Gram positive (S. aureus and three strains of Gram negative (E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) bacteria were used. Media specific for each strain were prepared (MSA for staphylococcus aureus, and MacConkey for Escherichia coli and klebsiella pneumonaie and nutrient agar for pseudomonas aerogenosa ) and on each labeled plate that contains the medium, was inoculated 40 μl of standardized broth culture of the bacteria.
Results
in pure form concentration plant leaves extracts of jasmin leaves shows 13mm zone of inhibition against Escherichia coli and against Methicillin Resistant staphylococcus aureus (MRSA) shows 6mm zone of inhibition.Against Klebsiella pneumonaie shows 6mm zone of inhibition.Against pseudomonas aerogenosa shows no zone of inhibition and labelled as resistant. In pure form concentration of plant leaves extracts of jasmin flower shows zone of inhibition 10mm against E.coli and shows 16mm zone of inhibition against Klebsiella pneumonaie.no zone of inhibition was found against methicillin resistant staphylococcus aureus and pseudomonas aerogenosa and labeled as resistant. Plant part of leaves extracts of Alium cepa(onion)showed no zone of inhibition against all resistant pathogens.
Conclusion
Antimicrobial resistance is alarming issue globally need to combat this issue. Our study shows that Jasmin leaves and jasmin flower and plant part of leaves extracts of Alium cepa (onion) showed no zone of inhibition against most of the resistant pathogen used in this study.But significant zone of inhibition was found aginst E.Coli, Klebsiella pneumonia.Similar studies need to conduct on resistant pathogen using different plat parts extract to analyse resistance patten of plant parts extracts against resistant pathogen so that better alternative should be detected.
To Cite this article:
Download
