PJB-2024-65
Phyllomicrobiome evaluation of Berberis baluchistanica from natural habitat through culture dependent and independent techniques
Zareen Gul
Abstract
Microorganisms inhabit nearly all parts of plants, residing as epiphytes and endophytes. Some bacterial association with plants is pathogenic, while others promote plant growth. Leaf-associated microorganisms of Berberis baluchistanica were examined at two sites (ZeroPoint ZBZP1, S1 ZBG2) of Ziarat by using culture-dependent and culture-independent methods. Results of culture dependent method showed that different phyla including Actinobacteriota, Proteobacteria, Bacteroidota, Firmicutes and Campilobacterota as well as Acinetobacter, Arcobacter, Alcanivorax, Arthrobacter, Brevibacillus, Bacillus, Flavobacterium, Gracilimonas, Idiomarina, Marinobacter, Pseudidiomarina, Pseudomonas and Sphingobacterium were among the several genera that could be grown. Furthermore, Arthrobacter, Alcanivorax, Bacillus spp., Bacillus cereus, Idiomarina, Gracilimonas, Marinobacter, Pseudidiomarina and Sphingobacterium were not examined in ZeroPoint ZBZP1 sample while Acinetobacter, Bacillus, Pseudomonas and Flavobacterium were evaluated in both samples. Several cultured bacterial communities could not be identified at the genus and specie levels. Results revealed that seven different phyla were categorized by culture independent technique such as Actinobacteriota, Campilobacterota, Bacteroidota, Desulfobacterota, Halanaerobiaeota, Firmicutes and Proteobacteria respectively. Genus level sequences classified overall 31 different bacterial genera and 21 various species could be sequenced. Sample from ZeroPoint ZBZP1 was predominated by Arcobacter suis and uncultured Halovibrio sample S1 ZBG2 was dominated by Bacillus selenitireducens, Alcanivorax venustensis, Arctic bacterium, Bacterium enrichment, Bacterium YC-ZSS-LKJ30, Bacterium YC-ZSS-LKJ80, Bioreactor metagenome, Gracilimonas amylolytica, Idiomarina aquatica, Marinilabilia salmonicolor, Marinobacter flavimaris, Pseudidiomarina homiensis, Robertkochia marina and few uncultured bacteria. Phyllosphere microbial communities exhibited the highest physiological potential and Gram-negative bacteria predominated more heavily in each sample than Gram-positive bacteria. Significantly higher number of bacteria were identified by culture-independent 16SrRNA gene sequencing.
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