PJB-2024-53
Enhancing Seed Germination in Podophyllum hexandrum Royle: Exploring Dormancy Release Treatments for Conservation and Cultivation Strategies
Zahoor Khan
Abstract
Podophyllum hexandrum Royle (P. hexandrum) is a medicinal plant of significant conservation value with potential for cultivation. However, the seeds exhibit strong dormancy, posing challenges to their propagation and utilization. This study aimed to assess the impact of various physical, chemical, and hormonal pretreatments on breaking seed dormancy and promoting germination in P. hexandrum, offering insights for effective conservation and cultivation practices. Additionally, the study investigated the effects of seed sterilization using different concentrations of mercuric chloride (HgCl2) on decontamination and germination. Results indicated that the 0.1% HgCl2 treatment was the most effective, resulting in a significantly higher germination percentage (85.43 ± 2.96%) and a lower contamination percentage (15.67 ± 2.96%) compared to other treatments. Various physical, chemical, and hormonal pretreatments were explored to overcome seed dormancy, with the highest germination percentage achieved under 5 mg L-1 GA3 (90.33%) for 24 hours. Hot water treatment for 60 seconds (80%) and cold water treatment for 24 hours (68%) also exhibited positive effects. The study revealed that GA3 and certain chemicals enhanced seed germination by overcoming barriers in the seed coat. Mechanical scarification (MES) contributed to improved germination by creating a small opening in the seed coat. These findings provide valuable insights for optimizing dormancy release treatments and enhancing seed germination in P. hexandrum, thereby contributing to conservation and cultivation efforts.
Keywords: Seed dormancy, Seed germination, chemical Pretreatments, Mechanical scarification
To Cite this article:
Download
