PJB-2024-43
Molecular analysis of genetic diversity in Tulip cultivars assessed by SRAP markers
Douwen Qin
Abstract
The taxonomy of Tulipa gesneriana L. poses a challenging problem, as traditional morphological methods no longer suffice to meet the demands. Nowadays, molecular marker technology has been increasingly utilized for population identification and classification, as well as for the analysis of genetic diversity and kinship relationships among tulip cultivars. To analyze the genetic diversity of various tulip cultivars, a study was conducted using the SRAP marker system to estimate kinship relationships among 40 cultivars. The results showed that out of 43 pairs of primers, 21 pairs with high polymorphism were selected, and 249 clear and stable bands were amplified, including 245 polymorphic bands. The percentage of polymorphism bands (PPB) is 98.39%. The genetic similarity index of 40 tulip cultivars was between 0.5020-0.8675. The genetic diversity parameters, including the number of alleles (Na), effective number of alleles (Ne), Nei's gene diversity index (H), Shannon’s information index (I), and polymorphic information content (PIC), were 1.9810, 1.5149, 0.3042, 0.4603, and 0.3212, respectively. This indicates that the genetic diversity of 40 tulip cultivars was rich. The cluster results in analysis and PCoA (principal coordinate analysis) divided tulip cultivars into two major groups A and B (‘Christmas Magical’, ‘Banja Luka’), with the first group (A) further divided into two subgroups A1 and A2 (‘Madame Lefeber’). In general, tulip cultivars have a high genetic diversity, which will provide a valuable basis for future breeding.
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