PJB-2024-14
Screening and identification of antagonistic fungi against pepper anthracnose and research on its pot-plant control effects
Xinyue Ma
Abstract
In order to enrich the high-quality biocontrol fungi resources for pepper anthracnose, this experiment obtained 282 fungal strains isolated and purified from soil by plate coating method, and screened 7 antagonistic fungi with obvious inhibitory strips by using the plate standoff method, and re-screened the inhibitory effect of the fungal fermentation broth by using the method of fungal plate containing poisonous medium.The results showed that the fungal strain A192 had a better inhibitory effect, and the inhibition rate of the rescreened fermentation broth was 61.29%. Morphological identification and molecular biological identification of the fungal strain, identified as Penicillium restrictum.Antagonistic fungal strain A192 fermentation broth of 13 kinds of pathogenic fungi inhibitory effect shows that the antagonistic fungal strain of a variety of pathogens have a certain degree of inhibition, on the Sclerotinia sclerotiorum, Fusarium oxysporum, Alternaria lternata, Phoma arachidicola, four kinds of pathogens. The inhibition rates of these four pathogens were high, 80.18%, 79.11%, 77.35% and 71.67%, respectively. A pot plant control effect test was conducted using the fermentation solution of fungal strain A192, and the control effect was 85.13%.
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