PJB-2024-5
PRELIMINARY ANALYSIS OF THE RESPONSE OF TOBACCO SA AND JA RESISTANCE-RELATED GENES TO EXSEROHILUM TURCICUM
LIN LIU
Abstract
Non-host disease resistance (NHR) is a broad-spectrum disease resistance produced by plants under the stimulation of non-adaptive pathogenic organisms, which has the characteristics of long-lasting disease resistance. In recent years, NHR has been considered as a new strategy for disease control. In this study, A. alternate and E. turcicum were inoculated into the main tobacco varieties HongHuaDaJinYuan (HD) and K326. The expression of disease resistance genes (NtAOS, NtJAZ, NtMYC2) in the upstream and downstream of Jasmonic acid Pathway (JA) and disease resistance genes (NtMAPK, NtPR1, NtPR2, NtPR11) in the upstream and downstream of Salicylic acid Pathway (SA) were analyzed by RT-qPCR. The changes of SOD, POD, PAL enzyme activity and H2O2 content were detected. The results showed that the expression levels of NtMAPK, NtPR1, NtPR2 and NtPR11 were up-regulated at 48h after inoculation with A. alternate and E. turcicum, the expression levels of HD and K326 were 1-13 times and 1-38 times higher than those without inoculation, respectively. The expression level of the gene after inoculation with E. turcicum was higher than inoculation with A. alternate; Similar to the SA pathway resistance-related genes, the expression levels of the JA pathway upstream resistance-related gene NtAOS and downstream related genes NtJAZ and NtMYC2 higher than those of the control and A. alternate stress groups at 48h after E. turcicum stress. The peak value of H2O2 content in the two varieties after E. turcicum stress was higher than that of the control and A. alternate stress at 12h; Moreover, the activity levels of SOD, POD and PAL at 48h after E. turcicum stress were higher than those under A. alternate stress. In summary, E. turcicum can induce non-host disease resistance in tobacco, enhance the ability of tobacco plants to resist host pathogens, and reduce or protect tobacco from pathogens to a certain extent.
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