PJB-2023-615
Genome-Wide Analysis of Cucumber (Cucumis sativus L.) Lines to Identify ZYMV Resistance Loci
ÖMER FARUK COŞKUN
Abstract
Control of virus diseases such as squash yellow mosaic virus (ZYMV), which causes serious yield and quality losses in cucumber, is not possible by using chemical pesticides other than eliminating virus vectors and using resistant varieties. The objectives of this study were to investigate structure of genetic resistance to ZYMV of cucumber for marker-assisted selection (MAS). Forty-eight resistant and 48 susceptible lines were selected from a germplasm of 600 cucumber lines. Bulk DNAs of resistant and susceptible lines were screened to find candidate marker(s) using ISSR, RAPD and a cleaved amplified polymorphism (CAP) primer, which targeted over 5000 cucumber loci. A total of 54 candidate marker loci were identified. In association analyses using TASSEL software, associated markers were detected. The results explained %78 of total ZYMV variation. The remaining 22% of the variation was possibly due to the presence of additional genes and environmental effects that caused lower repeatability.
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