PJB-2023-525
CRISPR/Cas Mediated Transgene-Free Strategies for Enhanced Virus Resistance in Agriculture: A Review
Amtul Wakeel Waheed
Abstract
Global food security is seriously threatened by the agriculture sector's substantial challenges caused by climate change and the introduction of dangerous viruses. Mega nucleases, zinc finger nucleases, and transcription activator-like effector nucleases are examples of earlier genetic modification techniques that allowed for targeted modifications but lacked the flexibility to target particular nucleic acid sites. But over the past nine years, the development of the CRISPR/Cas9 system has completely transformed how genome editing is done in a variety of plants. This review, focused on CRISPR/Cas9-mediated transgene-free strategies against DNA and RNA viruses that target host plant factors and virus resistance strategies that target the viral genome. In conclusion, CRISPR/Cas9 technology will give agriculturally significant crops a more robust and all-encompassing viral resistance.
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