PJB-2023-518
Numeric–Phenetic Study of Hyacinth Bean (Lablab purpureus (L) Sweet) from East Java Based on Morphology Character and DNA Fingerprinting Analysis
Elly Purwanti
Abstract
This research aims to conduct a Numeric – phenetic study of Lablab Purpureus (L) Sweet based on morphology character and DNA fingerprinting with cophenetic–correlation algorithm confirmation. L. Purpureus (L) Sweet samples were taken from 4 districts in East Java: Sumber, Lumbang, Bantaran, and Gunungtugel. Morphology selection character has resulted in four L. Purpureus (L) Sweet accession: Brown Seed White Flower (BrS-WhF), Black Seed, Purple Flower (BlS-PrF), Black Seed White Flower (BlS-WhF), and White Seed Purple Flower (WhS-PrF). DNA genome extraction is based on Blood Animal Plant DNA Preparation Kit. Ten RAPD primers were chosen for the amplification DNA genome, including OPA-6, OPA-8, OPA-10, OPA-20, OPC-19, OPD-8, OPD-12, OPE-8, OPE-15, and OPE-16. Cophenetic – correlation is used to analyze three clustering algorithms (Nearest Neighbor, UPGMA, Farthest Neighbor) and five similarity indexes (Simple Matching, Jaccard, Nei & Li, Sorensen, Yule). Dendrogram is constructed based on the optimum R-value using MVSP 3.1A. Lablab Purpureus (L) Sweet samples have shown morphology variation, mostly on flowers, pods, and seeds. DNA fingerprinting shows various band numbers with different molecular weights produced by RAPD primer. Most bands have molecular weights between 300BP-1000BP. A total of 87 bands, with 18 polymorphic bands, were found from RAPD analysis with an average percentage polymorphism of 31.15%. Cophenetic–correlation analysis resulted in the UPGMA algorithm, and the Jaccard similarity index is the best combination for dendrogram construction. Morphology character dendrogram has a slightly different topology compared to DNA fingerprinting dendrogram. It could be concluded that Numeric–phenetic study based on morphology character and DNA fingerprinting confirmed by cophenetic–correlation analysis has shown that the UPGMA algorithm and Jaccard similarity index is the optimum combination to analyze the diversity of L. Purpureus (L) Sweet accession from East Java, which resulted in the accession of this species has high diversity.
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