PJB-2023-517
MOLECULAR AND COMPUTATIONAL CHARACTERIZATION OF VARIATION IN TRANSLATION ELONGATION FACTOR METHYLTRANSFERASE EFM6 OF SORDARIA FIMICOLA AND ITS EFFECT ON POST TRANSLATIONAL MODIFICATIONS &a
Maimoona Ilyas
Abstract
Post-translational modifications (PTMs) are important alterations of the proteome that make species more compatible with changing environment. The current study was designed to reveal the importance of PTMs and genetic polymorphism of elongation factor methyltransferase 6 (EFM6) gene in six different strains of Sordaria fimicola through different molecular techniques and computational tools. The EFM6 gene is involved in the methylation of S-adenosyl-L-methionine (SAM) at the N-terminus of the protein which is an important modification for the survival of the species. S. fimicola strains were collected, three from South facing slope (S1, S2, S3) and three from North facing slope (N5, N6, N7) of Evolution Canyon, Israel. DNA was extracted from all six strains and sequences were obtained after amplification of genes. A total of 32 nucleotide variations in all six strains were observed compared to the reference Sordaria macrospora. There were 25 interstrain variations observed when strains were compared with each other along with 10 and 7 interstrain polymorphic sites in SFS and NFS strains respectively. Predicted putative 3D structure of EFM6 gene of S. fimicola revealed that it is consistent with known structure of SAM. Acetylation sites were predicted at 8 similar positions in both SFS and NFS strains. Glycosylation was predicted on asparagine residues, with N-glycosylation only on 1 site in all strains of S. fimicola while O-glycosylation was predicted on 12 different positions in SFS and 16 positions in NFS strains. Potential phosphorylation was determined with 7 and 13 sites in SFS and NFS strains respectively. Protein network analysis showed that EFM6 gene has 11 biologically active interactions with other genes that work as a group.This whole molecular and bioinformatics analysis generated valuable knowledge that stressed environmental conditions are responsible for genetic variations among species and despite inter-species heterogeneity and complexity the EFM6 domain is conserved in almost all eukaryotes.
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