PJB-2023-457
IN-SILICO ANALYSIS OF GERMIN AND GERMIN-LIKE PROTEIN FAMILY (BdGLPs) IN BRACHYPODIUM DISTACHYON
MUHAMMAD ILYAS
Abstract
Brachypodium distachyon germin-like proteins (BdGLPs) are members of cupin superfamily involved in the regulation of plant development and protection against stresses. These proteins are functionally unexplored. In-silico analysis of these proteins may lead to a better insight into their functional analysis. Therefore, the present study was aimed at characterizing structural, functional, and phylogenetic aspects, and expression patterns of 08 BdGLPs using computational tools. Physiochemical properties, 3D modelling of identified proteins, sites for phosphorylation and N-glycosylation, subcellular localization, phylogenetic relationship, domain composition, and conserved domains of 08 BdGLPs were predicted using in-silico approaches. Blast2GO and STRING v10 were used for functional characterizations of the BdGLPs. For assessing phylogenetic relationships, the MEGA6 tool was employed. Except for BdGLP3-6, the rest of BdGLPs showed similar physiochemical properties and subcellular localization. All BdGLPs were located on the same chromosome (3) except BdGLP2-1, which was present on chromosome 2 and showed a unique function “Remorin”, a plant-specific protein. This analysis will provide valuable insights into the potential utilization of BdGLPs in functional genomics research for dedicated biofuel crops, as well as temperate cereals and grasses.
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