PJB-2023-387
ANTIOXIDANT DIMENSION AND POTENTIAL AS MICROBIAL GROWTH INHIBITORS IN TWO HIMALAYAN ARTEMISIA SPECIES (ARTEMISIA MARITIMA L. AND ARTEMISIA INDICA WILLD.) CRUDE EXTRACTS FROM THE NORTHEASTERN (GILGIT-BALTISTAN), PAK
Hassam Rasheed
Abstract
Microbes in living organism are responsible to instigate various contagious diseases and the microbial resistance towards antibiotics and the evolution of new strains are of great apprehension these days. For this reason, bioactive compounds from Artemisia plants could be employed as natural agents to cope with microbial infections. This study assessed TPC and TFC with antioxidant and antimicrobial efficacies inextracts of two Himalayan endemic Artemisia species (Artemisia indica Wild. and Artemisia maritima L.) from Gilgit-Baltistan, Pakistan. TFC and TPC were assessed with aluminum chloride colorimetric and folin ciocalteu’s procedures and antioxidant efficacy was evaluated with TAC and FRSA-DPPH methods. Inhibitory activities of extracts against 4 bacterial strains were assessed with well diffusion technique. The maximum extract yield was attained with methanol in A. maritima (5.14g, 12.85%) and A. indica (4.19g, 10.47%), while lower yield was obtained with n-hexane in A. indica (0.51g, 1.29%). Maximum TPC and TFC in A. indica and A. maritma were obtained in the ethanol extracts. The ethanol extracts in both species overall displayed maximum DPPH percentage inhibition with maximum TAC in A. indica chloroform and A. maritima methanol extracts. The extracts of A. indica at 5-50 mg/ml exhibited inhibitory effects against bacteria with inhibition zones of 10 to 25 mm while A. maritima displayed inhibition zones of 8 to 23 mm. In the extracts of plants, highest growth inhibitions were obtained at 50 mg/ml against all strains. For the MICs, methanol and ethyl acetate extracts of both plants showed greater inhibition against P. aeruginosa then B. subtilis at lower concentrations. On the whole, the A. indica and A. maritima plant extracts are significant sources of essential bioactive compounds with pharmaceutical applications as natural antibacterial agents.
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