PJB-2023-382
Effect of culture filtrates of A.flavus on seed germination and seedling growth of sesame.
Raheela Rehman
Abstract
Sesame is highly susceptible to pathogenic fungus such as Aspergillus flavus. Effects of secondary metabolites of culture filtrates of A.flavus were assessed for activity on seed germination and seedling health of sesame seeds. Culture filtrate was prepared using PDB (Potato dextrose broth) and then inoculated with A.flavus. After 7 days of inoculation, healthy grown sesame seeds were inoculated with the spore suspention of A.flavus.Three isolates of A.flavus i.e. AFI,AFII and AFIII were inoculated on the sesame plants and the results were reported after 3, 5, 7 and 10 days. The overall result reported that the seeds treated with culture filtrates of A. flavus showed 30-50% reduction in growth day by day whereas the growth of control was increased up to 80- 100%. On the 10th day of observation, maximum germination reduction was reported by seed treated with AFI (60%) when compared to control. Seedling treated with culture filtrates of A. flavus showed various symptoms including brown lesions with yellow borders appeared. The illness then gradually extended from the leaf edges to the mid veins, causing defoliation, leaf yellowing, and brown streaks in the vascular tissues of the leaf. This is a baseline study to evaluate highly active metabolites which can be managed further to decrease sesame yield loss.
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