PJB-2023-379
MOLECULAR CHARACTERIZATION AND STUDY OF THE IMPACT OF POLYMORPHISM ON POST-TRANSLATIONAL MODIFICATIONS OF RKM1 IN VARIOUS STRAINS OF SORDARIA FIMICOLA.
Iqra Mobeen
Abstract
Post-translational modifications (PTMs), including phosphorylation, glycosylation, methylation, and acetylation are very important biological processes that alter the expression of proteins. In the current study, we have obtained a near-complete sequence coverage of RKM1-MTase and identified seven polymorphic sites, and for the first time carried out the detailed molecular characterization and traced the direct association of natural polymorphism on amino acid pickings/PTMs for SFS (S1, S2, and S3) and NFS (N5, N6, N7) strains of S. fimicola. 2D structures of protein showed differences in the sites of α-helix, β strand, and coil while 3D structures built by I-TASSER have shown differences in the loop regions. Ligands studies have shown that RKM1-MTase of S. cerevisiae carries 3 ligands, while SFS stains and NFS strains have 2 and 5 potential ligands, revealing its therapeutic relevance. We reported 52 phosphorylation sites for S. cerevisiae, while 47 and 51 phosphorylation sites for SFS strains, and NFS strains using NetPhos 3.1 server. RKM1-MTase carries 04 O-glycosylation (05 for SFS strains) sites and seven N-glycosylation residues for NFS strains and S. cerevisiae. Moreover, one lysine methylation for each strain wherein 21 and 23 lysine acetylation were observed for SFS and NFS strains, respectively. We have obtained differences in the number of modified sites, especially in phosphorylation, glycosylation, and acetylation sites among SFS and NFS strains and also in reference to S. cerevisiae. This difference is due to the natural genetic variations that we have observed in the protein sequence of RKM1 for these strains. This study concludes polymorphism alters gene expression and leads to the creation of diverse types of protein motifs/frontier molecules through PTMs that are specifically preferred by the organisms living under contrasting and stressed environmental conditions, which help them survive.
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