PJB-2024-1500
Assessment of antimicrobial and antioxidant properties of silver nanoparticles from Nepeta laevigata (D.Don) Hand. -Mazz. and Nepeta kurramensis Rech. f.
Memoona Almas, Sameen Jan and Zabta Khan Shinwari
Abstract
The present work focusses on antimicrobial and antioxidant activities of silver nanoparticles (AgNO3 NPs) from two important medicinal plants Nepeta leavigata and Nepeta kurramensis. The AgNO3 NPs antimicrobial results revealed that both the plants showed good potential against bacterial and fungal pathogens. The AgNO3 NPs from N. leavigata showed 20.5 ± 0.047, 19.8 ± 0.408 and 18.7± 0.037 mm zone of inhibition against Amanita muscaria, Fusarium oxysporum, Aspergillus terreus respectively at a concentartion of 11 µg/ml while the lowest zone of inhibition 7.93 ± 0.391 mm was given against fungal pathogen Puccinia graminis. Antifungal results given by AgNO3 NPs from N. kurramensis projected that they showed 20.16 ± 0.067, 19.6 ± 0.045 mm inhibition zone at a concentration of 11 µg/ml against Amanita muscaria, Fusarium oxysporum respectively while lowest inhibition zones 6.8 ± 0.048 and 6.3 ± 0.037 was given against Puccinia graminis, Geotrichum candidum. Three different concentartion of AgNO3 NPs from our research plants were tested against seven bacterial pathogens. Results revealed that AgNO3 NPs from N. leavigata exhibited 21.8 ± 0.065, 21.1 ± 0.05 and 19.6 ± 0.048 at a concentration of 7 µg/ml against P. aeruginosa, Listeria monocytogens and Salmonella typae respectively. Lowest zone of inhibition 16.7 ± 0.04 was given against Klebsiella pneumonia. The AgNO3 NPs from N. kurramensis showed highest zone of inhibition 21.8 ± 0.045 against P. aeruginosa while the lowest inhibition zone 16.8 ± 0.043 was given against E. coli at a concentration of 7 µg/ml. The antioxidant activities results showed that AgNO3 NPs from N. kurramensis extract had a higher scavenging effect than AgNO3 NPs from N. laevigata. AgNO3 NPs from N. laevigata demonstrated the maximum % of inhibition, measuring 47.17 ± 0.032 at a dose of 9 µg/ml. This was followed by 43.86 ± 0.023 % inhibition at a concentration of 7 µg/ml, whilst the lowest percentage activity was detected at a concentration level of 3 µg/ml, which was measured as 34.56 ± 0.028. The maximum scavenging inhibition given by AgNO3 NPs form N. kurramensis was 50.03 ± 0.073 % at a dose of 9 µg/ml, and it was followed by 46.7 ± 0.043 % inhibition at a concentration of 7 µg/ml
To Cite this article:
Almas, M., S. Jan and Z.K. Shinwari. 2024. Assessment of antimicrobial and antioxidant properties of silver nanoparticles from Nepeta laevigata (D.Don) Hand. -Mazz. and Nepeta kurramensis Rech. f. Pak. J. Bot., 56(1): DOI: http://dx.doi.org/10.30848/PJB2024-1(1)
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