PJB-2023-324
Effect of earthworm manure accompanied by white clover cultivation on metabolites of L. davidii var. unicolor bulbs and their inter-root soil
Jianming Wang
Abstract
In order to study the effects of earthworm manure accompanied by white plantain cultivation on the metabolites of L. davidii var. unicolor bulbs and its inter-root soil, four cultivation methods were set up: T1 (white plantain + earthworm manure), T2 (white plantain + sandy soil), T3 (earthworm manure) and T4 (sandy soil) with L. davidii var. unicolor as the test material, and by measuring the soil physicochemical properties, the The effects of each treatment on soil physicochemical properties were analyzed; based on LC-MC non-targeted metabolomics technology, the differences of metabolites of L. davidii var. unicolor bulbs in different treatments were analyzed and compared; based on 16S rRNA high-throughput sequencing technology and fungal ITS sequencing technology, the diversity and community distribution patterns of soil microorganisms between the roots of L. davidii var. unicolor in different treatments were investigated. The results showed that there were significant differences (p<0.05) in the metabolites of L. davidii var. unicolor bulbs under different treatments, and 15 metabolic pathways were enriched among L. davidii var. unicolor bulbs in treatments T1 and T4, of which six metabolic pathways had p-value values less than 0.05, and the differential metabolites were Phenylacetic acid, Phenylpyruvic acid, PE (15:0/16:1(9Z)), Acetoacetic acid; nine metabolic pathways were enriched between lily bulbs in T2 and T4 states, among which three metabolic pathways had p-value values less than 0.05, among which the differential metabolites were Imidazoleacetic acid ribotide, Glutathione L-2-Amino-4-methylenepentanedioic acid; six metabolic pathways were enriched between lily bulbs in T3 and T4, among which two metabolic pathways had p-value less than 0.05, among which the differential metabolites were PC(16:0/0:0), Biopterin; T1 treatment on organic matter, alkaline soluble N, fast-acting N, fast-acting K and fast-acting N and fast-acting K were significantly higher (P<0.05) than the (T2, T3, T4) treatments; the accompanying planting of white cheatgrass changed the microbial community structure of the inter-rhizosphere soil of L. davidii var. unicolor, and the T1 treatment significantly increased the relative abundance of Chloroflexi and decreased the relative abundance of Firmicutes, Actinobacteriota T1 treatment significantly increased the relative abundance of Chloroflexi and decreased the relative abundance of Firmicutes, Actinobacteriota, and Proteobacteria; the top 30 microorganisms in the total abundance of inter-rhizosphere soil of L. davidii var. unicolor. under different treatments formed two taxa, which were significantly correlated with soil physicochemical factors (P<0.05). The cultivation of white plantain with earthworm manure can improve its inter-root soil fertility, which plays a positive role in soil ecological balance and health, and provides theoretical guidance for the high-quality cultivation model of L. davidii var. unicolor.
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