PJB-2023-215
Phage combination therapies for bacterial wilt disease in plants
Taimoor Saddique
Abstract
Ralstonia solanacearum, which creates bacterial wilt disease, poses a serious danger to tomato crops all over the world. There are no known immunisations, and conventional therapies for the disease, like chemical 1s, are not usually successful. Phage therapy, which employs viruses that infect and kill bacteria, has recently come to be recognised as a possible substitute for managing the bacterial wilt disease. Phage combination treatments, in particular, have demonstrated encouraging outcomes in the management of the illness. The utilisation of phage combination therapy for the treatment of tomato bacterial wilt illness will be discussed in this review. The many techniques for isolating and describing phages, as well as the effectiveness of these treatments in managing the illness, will be discussed. Phage combination therapies present a promising strategy, but there are also issues that must be resolved before they are widely applied in agriculture. These difficulties include the emergence of phage resistance as well as the expense and logistical difficulties of mass-producing and using phages. It's likely that more investigation will lead to the development of fresh, cutting-edge phage treatments that are more productive, inexpensive, and available to farmers. This study looked at how well various bacteriophage combinations worked to prevent Ralstonia solanacearum infections in tomato plants. In both greenhouse and area studies over the course of a single crop season, the study discovered that increasing the number of phages in combinations decreased the incidence of disease by up to 80%. The fall in illness incidence was attributed to the reduction in pathogen mass, the choice of phage-resistant but low-rising pathogen strains, and the advancement of bacteria species antagonistic to R. solanacearum.. According to the study, some phage combinations may be effective as precise instruments to manage plant pathogenic bacteria without changing the rhizosphere microbiota that already exists. The article covers how to cure Ralstonia solanacearum, a Gram-negative bacteria that make bacterial wilt in crops, using 3lytic phages (RSA1, RSB1, and RSL1). The scientists discovered that treatment with RSA1 and RSB1, either separately or together, cautilised a fast decline in bacterial cell mass. But, about 30 hours after treatment, cells that were resistant to these phages started to form. Treatment with only RSL1 produced a reduced cell mass that persisted for a longer time. Pretreating tomato seedlings with RSL1 inhibited the proliferation and mobility of the root-inoculated bacterial cells, and no treats plants showed indications of wilting during the experiment. Also, it was discovered that RSL1 is relatively persistent in soil at high temperatures, and 4 months after infection, active particles were found. In order to effectively avoid wilting, the authors propose that RSL1 could be utilised as an alternative biocontrol approach that kills some but not all bacterial cells while allowing the bacterium and the phage to coexist.
To Cite this article:
Download
