PJB-2023-200
Comparative Assessment of Oil Biodegradation Potential of Bacterial Strains Pseudomonas aeruginosa and Enterobacter cloacae
MARIAM ZAMEER
Abstract
The biosurfactants are dynamic substances of living organisms and can be utalized in environmental cleaning by biodegradation and detoxification of industrial discharges and in the bioremediation of polluted soil. Presently, thirteen strains of bacteria were isolated from greasy contaminated soil and agro-industrial waste; molasses. Nine strains were screened from molasses and soil samples (M13, M7, M9, S1, S3, SC, SD, SE, and SF) which exhibited high emulsification index (E24) with olive oil, kerosene oil, cooking oil, diesel, mustard oil, and petrol. Isolated strains showed oil degradation potential on pH 5, pH 7 and at 37 °C with 1% oil concentration. Screened bacteria were found resistant to some heavy metals and antibiotics. Isolated bacteria were identified as Pseudomonas aeruginosa and Enterobacter cloacae using 16s rRNA sequencing. Biosurfactants extracted from isolated bacterial strains exhibited a resemblance with lipopeptide and glycolipids type of biosurfactants. The study signifies the identification of biosurfactants-producing bacteria and their potential to degrade the oil.
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