PJB-2023-150
Role of PGPR in the establishment, proliferation and biocontrol activity of Vesicular arbuscular mycorrhizae (VAM) and suppression of root rot phytopathogens
Mariyam Ahmed
Abstract
Symbiotic relationship of AMF with plants reported for a long time. AMF regulates growth and development of plants by colonizing under the cortical region of roots and promotes nutrient uptake especially phosphorus (P) and nitrogen (N) to plants. Spores of VAM isolated from mycorrhizosphere of healthy plants. Among identified VAM fungi, Glomus was found dominant, however, Acaulospora and Gigaspora were also recorded. For the establishment of intra radical colonization and proliferation of VAM fungi, endophytic fluorescent Pseudomonas (PGPR) strains were applied and indicated the biocontrol ability of VAM fungi with the induction of systemic resistance against root rotting pathogens. Six isolates of EFP-102, EFP-105, EFP-106, EFP-110, EFP-111 and EFP-112 were obtained from Agriculture and Biotechnology Laboratory Culture Collection Centre, and tested in vitro against four root decaying fungi viz., Rhizoctonia solani, Fusarium oxysporum, F. solani and Macrophomina phaseolina. In dual plate culture assays PGPR showed significant suppressive effect on root rot fungi. Culture filtrates of four screened strains of EFP tested by disc diffusion method against root rot. In vivo combined treatment of EFP isolates and VAM spores under screen house in sterilized soil showed improvement in plant growth and yield. Sterilized soil make a good impact on proliferation of VAM spores. EFP isolates, improves phosphorus and nitrogen uptake and upgrades systemic resistance. Laboratory based research on biocontrol to industry is a major aspect of our research studies, so the implementation of bio inoculants can be done easily. VAM is frequently used as bio inoculants. The extreme complexity of interactions that can occur in the rhizosphere is highlighted and some potential areas for future research are discussed briefly. Finally, the ability of PGPR to compete and successfully colonize the rhizosphere should be considered in the development and application of bio inoculants.
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