PJB-2023-116
AN INDIGENOUS WILD MUSHROOM LEPISTA SORDIDA MEDIATED SYNTHESIS OF SILVER NANOPARTICLES AND THEIR ANTIFUNGAL POTENTIAL
TAYYABA KHALIL
Abstract
The development of natural nanomaterials is of great interest to researchers involved in the biosynthesis of environmentally friendly nanoparticles. Macrofungal-assisted synthesis of nanoparticles has been reported to produce a large amount of protein with high yield and low toxicity in many countries but this is a neglected filed in Pakistan. The aim of this research was to investigate the potential of wild neglected mushroom Lepista sordida occurring in Pakistan to synthesize silver nanoparticles (AgNPs) through economical and eco-friendly methods and to evaluate their antifungal potential against pathogenic fungi. Novelty of this work is that this mushroom was examined first time for synthesis of its nanoparticles in Pakistan. Another significance of this investigation is that Lepista sordida was first time analysed through molecular analysis from Pakistan. Previously it was only described morphologically from this country. Due to their unique physical and chemical properties, silver nanoparticles (AgNPs) are increasingly used in various fields, including medicine, food, health, consumers and industry. The identification of mushrooms was carried out through morphological and molecular analysis. The synthesis of AgNPs (through Microwave assisted extraction method) was confirmed. These products were characterized by ultraviolet (UV)- visible spectroscopy, Scanning electron microscopy (SEM), and X-ray diffraction analysis. UV-visible spectrum for silver nanoparticles exhibited a broad absorption peak between 200–800 nm and showed a maximum absorption spectrum at 345 nm, which indicated the existence of AgNPs. SEM micrographic studies confirmed that synthesized AgNPs from Lepista sordida extract had smooth hexagonal morphology in the nano-range. The antifungal activity was performed against the pathogenic micromycetes species (Aspergillus flavus and Alternaria alternata) to check the maximum inhibitory effect of AgNPs against these species. The AgNPs synthesized with fungus extract inhibited the growth of A. flavus and A. alternate by 97.3 and 91.75% at the concentration of 10µg/L. This indicated that the synthesis of AgNPs using fungus had the capability to control pathogen's growth with low toxic rate and good biocompatibility mode. It is concluded from the present study that synthesized AgNPs have good potential to be used as antifungal agents against fungal pathogens. This work can be helpful in future projects related to development of nanofungicides against fungal pathogens.
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