PJB-2023-32
Isolation, Identification and Characterization of Rhizosphere and Phyllosphere Microbiomes from Healthy and Huanglongbing (HLB) Infected Citrus Cultivars in Punjab, Pakistan
Wajeeha batool chaudhary
Abstract
Different types of microorganisms inhabit the rhizosphere and the phyllosphere, where they perform various but distinct ecological tasks. The rhizosphere and phyllosphere of citrus plants were studied for microbial growth using culture-dependent methods from the citrus samples collected from Punjab, Pakistan. Fifty soil and fifty leave samples containing healthy and Huanglongbing (HLB, citrus greening) infected samples were collected from the orchard of Citrus research institute, Sargodha, Pakistan. The presence of huanglongbing (HLB) infectious pathogen was detected by amplification of 1174 bp fragment from 16S rDNA. Sixteen bacterial isolates were identified from the rhizosphere, and six bacterial isolates from phyllosphere. The most prominent taxa present in healthy rhizosphere of citrus were Citrobacter freundii (C. freundii), Serratia marcescens (S. marcescens), Enterobacter aerogenes (E. aerogenes), Proteus valgaris (P. valgaris), Enterobacter cloacae (E. cloacae), Bacillus cereus (B. cereus), Bacillus anthracis (B. anthracis), Bacillus subtilis (B. subtilis), Enterococcus faecalis (E. faecalis), Klebsiella pneumonia (K. pneumonia) and Staphlococcus epidermidis (S. epidermidis) while, Escherichia coli (E. coli), Shigella flexneri (S. flexneri), Pseudomonas aeruginosa (P. aeruginosa), Salmonella typhi (S. typhi) and Staphylococcus aureus (S. aureus) were found in HLB infected plants. P. valgaris, E. aerogenes and K. pneumonia were abundant in phyllosphere of healthy citrus plants, while E. coli, P. aeruginosa and S. typhi were dominant in HLB-infected samples. Bacterial communities in healthy citrus cultivars were reported as plant growth promoting bacteria, while those identified in HLB-infected citrus plants were considered plant pathogenic bacteria.
Keywords: Huanglongbing, Candidatus liberibacter asiaticus, Rhizosphere, Pathogen
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