PJB-2023-15
PHYTOPHARMACOGNOSTIC SCREENING AND FOURIER TRANSFORM-INFRARED (FT-IR) ANALYSIS OF THE ARIEAL PARTS OF CROTON BONPLANDIANUS
Hina Yasin
Abstract
Croton bonplandianus belongs to family Euphorbiaceae and it is an important traditional plants. Phytopharmacological analysis indicates that the leaves of C. bonplandianus significantly exhibited wound healing properties that may be due to the presences of antioxidant enzymes and rutin etc. Present investigation comprises on organoleptic, microscopic, physicochemical and phytochemical analysis of aerial parts of this plant. Fourier Transform Infrared (FTIR) Spectrophotometer was also performed using leaves extract for the evaluation of peak value with various functional groups. Preliminary phytochemical screening was performed for the identification of medicinally valuable constituents. Fluorescence analysis was carried out to observe the behavior of powdered material with different reagent under UV light. All these parameters are used as tool for proper identification, authentication and standardization of plant material. Histological examination of leaves of C. bonplandianus showed that the mid rib considerably curved upper epidermal and U-shaped lower epidermal cells while unicellular trichomes were short and branched at the base. Vascular bundle packed and conjoined. Wavy parenchymatous cells abundantly present with parasitic stomatas. The identical features of bark of C. bonplandianus were physically observed that showed wavy outline, barrel shaped, thick walled, compactly packed. Powdered leaves were revealed the presences of starch grain, stomata, parenchymatous cell and epidermal cells patches along with trichomes. Phytochemical studies indicate the presences of alkaloids, phenol, carbohydrates, steroids, saponins, proteins, and amino acids. The FTIR method was through spectrophotometric technique for the detection of functional groups and peaks values. This analysis was generated the FTIR spectrum profile of C. bonplandianus. This research would assist in future to reduces the chance of contamination, adulteration, improper &/or incorrect identification and purification, although supports the isolation of medicinally active constituents of C. bonplandianus.
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