PJB-2022-569
PRODUCTION OF VIRUS FREE SEED POTATO OF COMMERCIALLY GROWN CULTIVARS IN KHYBER PAKHTUNKHWA THROUGH MERISTEM TIP CULTURE AND MICROPROPAGATION TECHNIQUES
MAHNOOR BALOCH
Abstract
Potato cultivars commercially grown since long in plains and hills of Khyber Pakhtunkhwa, are now heavily infected with single or multiple virus infection and subsequently degenerated. Incidence of six prevalent potato viruses (PLRV, PVY, PVX, PVS, PVM and PMTV) has been investigated in 10 cultivars through double antibody sandwich (DAS) and triple antibody sandwich (TAS)-Enzyme Linked Immuno-sorbent Assay (ELISA). All commercially grown potato cultivars tested were heavily infected with one or more prevalent potato viruses. Cultivars such as Cardinal, Santé, Asterix and Kuroda showed the highest incidence of potato viruses and hence selected for cleaning of potato viruses through meristem tip culture and micropropagation. Meristem tip containing apical dome along with few leaf primordia were excised and cultured on MS media under aseptic conditions in tissue culture conditions. The cultures were incubated for 6-8 weeks for the plantlet formation. From each cultivar, 27 plantlets were selected and transferred to the sterilized soil for mini-tuber production. Finally, 21, 27, 18 and 16 mini-tubers were harvested from in vitro plantlets of cultivar Cardinal, Santé, Asterix and Kuroda, respectively. All the mini-tubers raised by meristem tip cultured plantlets were virus-free when tested through ELISA. Mini-tubers were stored at 4°C for further process.
To Cite this article:
Download
