PJB-2022-566
MARKER-TRAIT ASSOCIATION IN PAKISTANI WHEAT GERMPLASM USING SSR MARKERS FOR DROUGHT TOLERANCE
Nadia Khan
Abstract
Abiotic stress severely limits the production of wheat globally. As Pakistan climatic condition range from arid to semi-arid, abiotic stresses such as heat, drought, and salinity has drastic effect on cultivation of staple crops like wheat. Development of abiotic stress tolerant varieties can be the most practical way for enhancing wheat production. For generating tolerant cultivars, marker assisted selection can be the most time-efficient, labor-saving and more productive than traditional breeding methods. Thirty-four wheat varieties were collected from different regions of Pakistan and analyzed using twelve SSRs for drought tolerance. The genotypes were associated with agronomic and seedling traits. Significant (P < 0.001) marker traits association were observed for root length, shoot length, fresh weight, dry weight and grain number. Genotyping with a limited number of markers is now replaced by using multitudes of markers providing, rapid, inexpensive, and authentic data for wheat breeding. This technique has revealed unique perspective for marker-assisted selection in genomic selections in wheat. These findings are highly useful and reveal functional diversity among wheat germplasm with varying response to abiotic stress.
Keywords: abiotic stress, agronomic traits, molecular marker, marker-trait, seedling traits, wheat
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