PJB-2022-542
Ecotoxicological Effects of Surbex-Z on Seed Germination and Seedling Development of Chickpea Seeds (Cicer arietinum L.)
Hafsa Razzaq
Abstract
Using early growing and developmental indices of chickpea, such as seed germination, shoot height and root length and toxic effects of Surbex-Z with therapeutic action on chickpea (Cicer arietinum L. from family Papilionaceae) were investigated as an example in order to assess ecological risk of pharmaceutical compounds entering into agricultural ecosystems. To perform this research chickpea seeds and Surbex-z tablets were purchased. The seeds were surface-sterilized in 3% (v/v) H2O2 for 5 min and then rinsed with deionized water. After rinsing off, seeds were soaked into water overnight. Next day, Seed germination experiment was performed on filter papers placed in Petri dishes and moistened with 5.0 mL solution of Surbex-Z. Controls were prepared by moistening the filter papers with 5 mL deionized water only. Seeds were considered to have germinated when both the plumules and the radicles were over 2 mm. The exposed experiment was finished after 21 days. The concentration of solution prepared with Surbex-Z is increases with 1.5, 2.5, 6.6, 10.2, and 20.00 mg/L for 21 days. All treatments were replicated three times to minimize experimental errors. The findings demonstrated that when Surbex-Z concentration increased, chickpea shoot elongation decreased and root elongation increases significantly. The most sensitive plant tissues to Surbex-Z were chickpea shoots. However, the activity of peroxidase in chickpea shoots decreased significantly after the 14-day exposure, which indicated the antioxidative defensive system in chickpea shoots was damaged by Surbex-Z.
Key words: Ecological risks, pharmaceutical compounds, Agricultural ecosystem, Toxicity, inhibition.
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