PJB-2022-520
AMMONIUM BROMIDE ENHANCES ORGANOGENESIS IN SYNGONIUM PODOPHYLLUM AND PICLORUM WITH 2, 4-D ACTIVATES SOMATIC EMBRYOGENESIS IN SYNGONIU MAURITUM
Venugopal Rajanbabu
Abstract
Micropropagation is a promising way to overcome difficulties such as time consumption, maintenance of plant uniformity and disease infection. An optimized method of micropropagation by direct organogenesis in Syngonium podophyllum and somatic embryogenesis in S. auritum has been demonstrated here. Direct organogenesis through the formation of auxiliary buds in S. podophyllum from shoot tip and nodal explants has been observed in modified MS media with 1 mg l-1 BAP and 0.1 mg l-1 NaH2PO4. In addition, the presence of 0.1 mg l-1 ammonium bromide consistently enhanced the proliferation rate up to five subculture cycles of shoot proliferation. Regeneration through somatic embryogenesis was observed in S. auritum in the presence of piclorum and 2,4-D in modified MS nutrient medium. Embryogenic calli were formed in the stem explant devoid of nodes in S. auritum in MS media with 10 mg l-1 picloram and 1 mg l-1 2,4,D. The embryogenic calli regenerated into full plants when subcultured in modified MS medium with 2 mg l-1 NAA and 1 mg l-1 IAA. Taken together, ammonium bromide along with NaH2PO4 increased the direct organogenesis of S. podophyllum, and piclorum with 2,4-D enhanced regeneration through somatic embryogenesis in S. auritum.
To Cite this article:
Download
