PJB-2022-514
CHARACTERIZATION OF ANTIFUNGAL METABOLITES FROM BACILLUS STRAINS HAVING BIOCONTROL POTENTIAL THROUGH LC-MS-MS TECHNIQUE
SHEHZAD MEHMOOD
Abstract
Biopesticides are safe, target specific, biodegradable, and eco-friendly. Bacteria have been characterized as biocontrol agents and to improve growth of plants through several mechanisms. In the current study, two previously isolated Bacillus strains including PM31 (Bacillus sp. PM31) and PM32 (Bacillus subtilis PM32) antagonistic to Fusarium solani and Rhizoctonia solani respectively were characterized for antifungal metabolites through Liquid Chromatography-Mass Spectrometry (LC-MS-MS). A total 20 antifungal metabolites (mainly included fengycin, pyrrolnitrin, iturin, bacillomycin, kurstakins, threonine and phenazine-1-carboxamide) were detected in strain PM31, whereas Bacillus subtilis PM32 produced 21 antifungal metabolites (mainly included lichenysin, bacillibactin, kinetin, phenazine-1-carboxamide and indole-3-carboxylic acid). A LC-MS-MS profile of respective strains also confirmed PGP-metabolites (included 2'-deoxyguanosine, glaucinea, mitsoamide) and (caffeic acid, albuterol, solasodine). Results of the above LC-MS-MS based metabolite profiling of both strains conferred their potential to be act as biopesticides as well as biofertilizers for sustainable production of crops.
To Cite this article:
Download
