PJB-2022-507
Screening and identification of Antagonistic Fungi against Tomato Early Blight and research on its pot-plant control effects
Xilin Sun
Abstract
According to some studies, Alternaria alternata has gradually risen to become the dominant pathogen in tomato early blight, which is one of the most widespread and serious diseases caused by Alternaria spp.. In order to enrich the resources of high-quality biocontrol strains, 8 antagonistic fungus strains with evident inhibition zones were first screened from the soil by dual culture assay, and then the effect of their sterile fermentation broth against A. alternata was re-screened by mycelium growth rate method. The results showed that strain L131 functioned well, with a primary screening inhibition zone of 5.54mm and a re-screening antifungal rate of 66.41%. L131 was identified as Penicillium citrinum after morphological and molecular biological identification. In addition, we discovered that the sterile fermentation broth of L131 could inhibit numerous pathogenic fungi to varying degrees. Among them, the inhibition rates of Sclerotinia sclerotiorum, Rhizoctonia solani, Exserohilum turcicum and Fusarium graminearum were 100%, 96.42%, 88.01% and 83.66%, respectively. Finally, we used the fermentation broth of strain L131 to conduct a pot control effect test, and the control effect was 85.13%.
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