PJB-2022-492
Active Compound Analysis of Ethanolic Extract of Roselle Calyces (Hibiscus Sabdariffa L.)
S A Sindhu Bharadhi
Abstract
Roselle (Hibiscus sabdariffa L.) is a comestible plant known for its fleshy red calyces that is used in making wide range of foods such as wine, juice, jam, syrup, pudding, cakes, ice cream and herbal tea. The anti-bacterial, diuretic, anti-oxidant and anti-mutagenic effects of the roselle calyces are also well-known. It is high in vitamins, minerals and bioactive substances such as organic acids, phytosterols and polyphenols and because of its extensive pharmacological potential, it has long been used as folk medicine to treat common cold. GC-MS is the best technique to identify the compounds present in the sample by mass spectra data obtained from pure available standards injected under the same conditions. In this study, the GC-MS technique was used to validate the pharmacological potential of Hibiscus sabdariffa by identifying the chemicals found in its calyces. The extract was prepared by soaking dry calyx powder sample in methanol overnight and the ethanolic extract was then analysed using GC-MS. Flavonoids, tannins, phenols, saponins, alkaloids, glycosides, terpenoids and steroids were found in the ethanolic extract of Hibiscus sabdariffa calyces. The existence of twenty-six bioactive chemicals was discovered by GC-MS analysis, including phthalic acid, astoxanthin, lutein, lycoxanthin, 3-Pyridinecarboxylic acid, rhodoxanthin, molybdenum and hexadecenoic acid. The presence of some of these bioactive chemicals has been used to support scientific evidence for the plant's anti-ageing, anti-oxidant, anti-hypertensive and anti-inflammatory capabilities.
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