PJB-2022-488
Optimization of an Ultrasound-Assisted Extraction method for the extraction of Phenols, Flavonoids and antioxidant compounds from Moringa Leaves (Moringa oleifera Lam.) var. PKM 1
T Arumugam
Abstract
Ultrasound-assisted extraction is one of the quantum leaps in extraction techniques that provide effective outcomes based on various parameters such as amplitude, temperature, time, frequency, intensity, solvent ratio, preparation process and quantity of solvent. The main objective of this experimental trial was to assess the impact of three factors including Amplitude, temperature and extraction time each of which consists of four levels of response on extraction yield (EY), total phenol content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA) by using Response Surface Methodology (RSM). The experimental trial was conducted with a range of independent variables such as 60-80% of amplitude, 25-45ºC of extraction temperature and 20-40 min of extraction time. The RSM design of this research trial employed 20 runs constructed by Central Composite Design (CCD). The output from the optimized extraction technique showed that the percentage of extraction yield was about 12.78 – 23.28%, total phenol content varied from 100.12 – 149.22 GA mg/g, total flavonoid content ranged from 14.43-20.19 QE mg/g and percentage of radical scavenging activity varied from 58.87 – 85.08 %. Furthermore, higher biomolecules (EY, TPC, TFC, and RSA) from the Moringa dried leaves were obtained in higher quantities with the increase in amplitude power along with the increase in extraction temperature.
To Cite this article:
Download
