PJB-2022-475
GENOME-WIDE IDENTIFICATION AND EXPRESSION ANALYSIS OF AUTOPHAGY GENES IN CUCUMIS SATIVUS UNDER ABIOTIC STRESS
Yue Yang
Abstract
Autophagy is an evolutionarily conserved physiological and developmental process in eukaryotes. In this process, damaged proteins in cells are degraded and cytoplasmic materials recycled. Cell autophagy has multiple functions in plants, it involved in growth, development, senescence, and responses to biotic and abiotic stress. So far, thirty three autophagy genes (ATG) have been found in rice, and more than 30 autophagy-related genes have been found in Arabidopsis, tobacco and corn. We explored the ATG gene family in the whole genome of Cucumis sativus based on bioinformatics methods and systematically analyzed the structure, conserved motifs, expression and phylogeny relationship of these ATG genes. Quantitative RT-PCR (qRT-PCR) was performed to detect the relative expression level of the CsATG genes in roots, stems and leaves of C. sativus under abiotic stress or treated with salicylic acid (SA) and Methyl jasmonate (MeJA). A total of 20 putative ATG genes were identified in the cucumber genome. Gene duplication analysis showed that both fragmented and tandem duplication played vital roles in the amplification of cucumber ATG gene family. Gene expression analysis showed that 16 CsATG genes were induced by SA treatment, and 16 CsATG genes were down-regulated by MeJA treatment. Under high salinity stress, 10 CsATG genes were induced in roots. Under drought stress, 16 CsATG genes were induced in roots. Under carbon starvation stress, all of 20 CsATG genes were induced to express in leaves, suggesting that cell autophagy has a potential role in nutritional starvation tolerance.
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