PJB-2022-464
PHARMACOGNOSTIC, PHYSICOCHEMICAL AND PHYTOCHEMICAL STUDIES OF A POLYHERBAL ANTI-GOUT REMEDY AND ITS INGREDIENTS
Ayisha Shaukat
Abstract
A traditionally used in-house prepared holistic polyherbal anti-gout remedy and its ingredients were investigated to get morphological, phytochemical and physicochemical profiles. The macroscopic feature indicated holistic remedy powder was brown in color and slightly bitter in taste. Its microscopy revealed the presence of the following: pigmented sclerenchymatous cells and vascular bundles, mucilage matrix and tannin-filled cellular debris, broken fragment of bast fiber, un-differentiated cells with tannin contents and solitary starch grains, xylary fibres, ovoid starch grains assimilated starch granules, undifferentiated sclerenchymatous cells with ovoid starch grains, bast fibre, broken ends of vascular bundle and cuboidal calcium oxalate crystals. In powder form, the remedy showed 16.75% total ash, 10.35% acid-insoluble ash, 8.08% water- soluble ash, 14.35% sulfated ash, 3.25% moisture, 10.35% alcohol extractives, 8.08% water extractives, swelling index (4.0 mL/g) and foaming index (< 100). The remedy contained 29.73 ± 0.3076, 79.0 ± 0.0002 and 65.694 ± 0.0444 total proteins, total lipids and total carbohydrates, respectively. The remedy further contained 138.51 ± 2.52, 35.324 ± 0.8039, 50.067±5.93, 50.146±0.811, 99.13 ± 0.115 mg/g total polyphenols, total flavonoids, total alkaloids, total tannins and total glycosaponins, respectively. The data generated from morphological, physicochemical and phytochemical features of the remedy helps in standardization of remedy.
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