PJB-2022-457
Establishment of direct organogenesis and efficient regeneration system of Pinellia ternate “zhenzhubanxia” leaves in vitro
Yan Ren
Abstract
We chose leaf as the explant and MS medium as the basic medium to establish the rapid regeneration system of Pinellia ternata (Thunb.) Breitenb “zhenzhubanxia”. The most suitable hormone types and their mass concentration ranges for the in vitro growth of P. ternate were selected by the single factor experiment. Subsequently, the complete combination and orthogonal experiments were designed to study the effects of different hormone combinations on tuber formation and plant regeneration. Although all hormones were beneficial to the growth of materials, 6-BA had the most significant effect on tuber direct generation, low concentration of 2,4-D made leaf forms callus, NAA and IBA made plantlet form adventitious roots. Meanwhile, the combination of 6-BA and NAA was beneficial to the occurrence of micro-tubers. In the L9 (34) orthogonal experiment of leaf, the MS medium with 6-BA 0.05 mg·L-1, NAA 0.1 mg·L-1, KT 0.5 mg·L-1 obtained the optional proliferation coefficient and rooting plantlets, the proliferation coefficient reached about 64.56. In addition, each leaf could form 13.5 micro-tubers and 18.39 petioles on average, tubers and petioles could all be transferred into the above medium as materials, so that the proliferation coefficient reached about 116.45. Meanwhile, the rooting rate was 100%, and the transplanting survival rate of plantlet was also 100%. An efficient artificial propagation system of P. ternata was established, and its proliferation coefficient was much higher than that of other species in the same genus, which realized the simultaneous culture of propagation and rooting. This system can cultivate seedlings with stable heredity and good quality to improve the artificial planting efficiency and solve the market demand and quality problems. At the same time, the rapid propagation system of P. ternata with high propagation coefficient can also provide experimental reference for artificial rapid propagation of Pinellia and other species of Araceae.
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